3/16/2024 0 Comments What is nod scid![]() ![]() Exploring the NK cell platform for cancer immunotherapy. ![]() Why natural killer cells in triple negative breast cancer? World Journal of Clinical Oncology. Values of ∗∗ P < 0.01 were considered statistically significant. FCM histograms show representative data of three independent experiments. (g) Statistical analysis of the FCM data for the IFN- γ expression on DX5 + populations of the splenocytes from poly(I:C)-treated mice. (f) Representative FCM data show the IFN- γ expression gated on DX5 + populations of the splenocytes from poly(I:C)-treated mice. (e) Statistical analysis of the FCM data for the IFN- γ expression on splenic DX5 + populations. (d) Representative FCM data show the IFN- γ expression gated on DX5 + populations of the splenocytes from naïve mice. (c) Statistical analysis of the ELISA data for IFN- γ in the culture supernatant. (b) Statistical analysis of the FCM data for IFN- γ and DX5 expression. ![]() (a) Representative FCM data show IFN- γ in and the expression of DX5 on the splenocytes from CB17/SCID and NOD/SCID mice. The splenocytes from naïve (a–e) or poly(I:C)-treated (f, g) mice were cultured with cytokines for 48 h. Caution should be taken when considering the use of NOD/SCID mice as an NK-deficient model.Īssay of IFN- γ in cells and cell culture supernatant in vitro. There was no significant difference in the proportion of splenic NK cells between CB17/SCID and NOD/SCID mice, and the function of NK cells was only partially compromised in NOD/SCID mice. After in vitro stimulation with cytokines, the splenocytes from CB17/SCID mice showed higher IFN- γ production than those from NOD/SCID mice however, NK cells did not. The splenocytes from CB17/SCID mice showed higher cytotoxicity than those from NOD/SCID mice, while the cytotoxicity of purified NK cells basically did not differ between the two strains. Moreover, the NKG2D and Ly49A levels in NK cells from NOD/SCID mice were higher than those from CB17/SCID. The perforin levels in NK cells were similar between the poly(I:C)-treated CB17/SCID and NOD/SCID mice, while the granzyme B and NKG2A/C/E levels in NK cells from NOD/SCID mice were significantly lower than those from CB17/SCID mice. The proportion of splenic NK cells did not differ significantly between NOD/SCID and CB17/SCID mice. Splenocytes from naïve or poly(I:C)-treated mice were isolated for phenotyping and analysis of cytotoxicity-related molecules and inhibitory receptors for cytotoxicity assay, purified NK cells were also used. However, the actual status of NK cells in NOD/SCID mice and CB17/SCID mice in comparison with that in BALB/c mice has not been sufficiently evaluated. NOD/SCID mice have been used as a model of this type in research. Natural killer (NK) cell-deficient mice are useful models in biomedical research. ![]()
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